E-cigarettes use aka vaping is being considered as an alternative to quit cigarette smoking while long-term effect of vaping on lung pathophysiology are unknown. Similarly vaping has been promoted and considered a safer alternative to replace conventional combustible cigarette smoking during pregnancy, hence needing further assessment. Dr. Sharma’s research team used models of in utero vaping exposure in mice and tested whether vaping during pregnancy affects offspring lung health and risk of developing allergic asthma. Offspring born to mothers who were exposed to e-cigarette vapor when challenged to ovalbumin allergen resulted in enhancement of features of allergic asthma. Offspring had greater Th2 inflammation in their lungs and were also characterized with increased mucus in the airways leading to exaggerated airway hyperresponsiveness (AHR) to a contractile agonist methacholine. These features mimic hallmarks of asthma in humans. Mechanistically, vaping perturbed mitochondrial function in isolated human airway cells and resulted in impairment of airway cell homeostasis characterized by increased cell-death and accumulation of fibrotic markers in vitro. Research established that some of these damaging effects in vivo were reliant on nicotine content in the vaping liquid while non-nicotine dependent detrimental effects were observed in isolated human airway cells (in vitro). These results suggest that maternal use of vaping may not be a safer alternative to cigarette smoking and at the same time establish that non-nicotine containing e-cigarette liquids could also pose equal risk. Caution therefore must be warranted in promoting these agents as safe smoking cessation aids in pregnancy and alternative smoking cessation strategies may be more appropriate to consider.